Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Distinct roles of histamine H1- and H2-receptor signaling pathways in inflammation-associated colonic tumorigenesis
doi: 10.1152/ajpgi.00212.2018
Figure Lengend Snippet: Antibodies and their applications used in this study
Article Snippet: Dimaprit dihydrochloride was obtained from Biotrend (Destin, FL), and 2-pyridylethylamine dihydrochloride was purchased from Tocris (Minneapolis, MN). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Name Company Clone Number or Catalog Number Applications and Dilutions H1R Santa Cruz Biotechnology P-20 Fluorescent staining: 1:200; immunoblot: 1:500 H2R Santa Cruz Biotechnology M-19 Fluorescent staining: 1:200; immunoblot: 1:500 Human H1R Elabscience ESAP13556 Fluorescent staining: 1:400; immunoblot: 1:1,000 PCNA Santa Cruz Biotechnology PC10 Fluorescent staining: 1:400 β-Actin Santa Cruz Biotechnology 11B7 Immunoblot: 1:500 Phospho-ERK Cell Signaling D13.14.4E Fluorescent staining: 1:300; immunoblot: 1:1,000 Phospho-p38 Cell Signaling D3F9 Immunoblot: 1:1,000 Phospho-JNK Cell Signaling 81E11 Immunoblot: 1:1,000 Phospho-p65 Cell Signaling 93H1 Immunoblot: 1:1,000 Total ERK Cell Signaling 137F5 Immunoblot: 1:1,000 BrdU Accurate OBT0030 Histochemical staining: 1:200 Open in a separate window H1R and H2R, H1 and H2 receptor, respectively; PCNA, proliferating cell nuclear antigen; BrdU, bromodeoxyuridine.
Techniques: Staining, Western Blot